Skip to Main Content


Jaundice is the most common transitional finding, occurring in 60–70% of term newborns and ~80% of infants born prematurely. An elevation of serum bilirubin concentration >2 mg/dL is found in virtually all newborns in the first several days of life. Jaundice becomes clinically apparent at serum bilirubin concentration of >5 mg/dL.


Bilirubin is the end product of the catabolism of heme derived primarily from the breakdown of red blood cell hemoglobin. The rate-limiting step in this production is the oxidation of heme to form a green pigment called biliverdin, a process controlled by the enzyme heme oxygenase. Each molecule of heme catabolized results in equimolar quantities of bilirubin and carbon monoxide. Other sources of heme include heme-containing proteins such as myoglobin, cytochromes, and nitric oxide synthase. Bilirubin exists in several forms in the blood but is predominantly bound to serum albumin; other compounds, such as drugs and metal ions, may compete with bilirubin for albumin binding sites. Elevated concentration of free unconjugated bilirubin, and possibly other forms, can enter the central nervous system (CNS) and become toxic to cells. The precise mechanism is unknown.


Inside liver cells, unconjugated bilirubin is bound immediately to intracellular proteins, the most important one being ligandin. It is then converted into an excretable and soluble form through the process of conjugation that consist of the transfer of one or two glucuronic acid residues from uridine diphosphoglucuronic acid (UDPGA) to form a monoglucuronide or diglucuronide conjugate. Uridine diphosphoglucuronyl transferase (UDPGT) is the major enzyme involved in this process. Conjugation is impaired in newborns due to reduced UDPGT activity and a relatively low level of uridine diphosphoglucuronic acid. Conjugated bilirubin is water soluble and can be excreted in the urine, but most of it is rapidly excreted as bile into the intestine. Conjugated bilirubin is further metabolized by bacteria in the intestine and excreted in the feces.


Hyperbilirubinemia presents as either unconjugated hyperbilirubinemia or conjugated hyperbilirubinemia. The two forms involve different pathophysiologic causes with distinct potential complications. In contrast to unconjugated hyperbilirubinemia, which can be transient and physiologic in the newborn period, conjugated hyperbilirubinemia is always pathologic. See Chapter 92 for a discussion of unconjugated hyperbilirubinemia.


  1. I. Definition. Conjugated hyperbilirubinemia is defined as a conjugated bilirubin concentration of >1.5–2 mg/dL or more than 10–20% of total serum bilirubin (TSB). It is the biochemical marker of cholestasis and a sign of hepatobiliary dysfunction. Unlike physiologic unconjugated hyperbilirubinemia, typically known as "physiologic jaundice of the newborn," it is important to emphasize that there is no physiologic conjugated hyperbilirubinemia. Chapter 50 provides information on the rapid "on-call" assessment and management.

  2. II. Incidence. Conjugated hyperbilirubinemia affects approximately 1 in every 2500 infants and is less common than unconjugated hyperbilirubinemia.

  3. III. Pathophysiology. Normal bile production involves two main processes: bile acid uptake by the hepatocytes from the blood, and bile excretion into the biliary canaliculus. Bile uptake from the blood is an active process facilitated by two ...

Want remote access to your institution's subscription?

Sign in to your MyAccess profile while you are actively authenticated on this site via your institution (you will be able to verify this by looking at the top right corner of the screen - if you see your institution's name, you are authenticated). Once logged in to your MyAccess profile, you will be able to access your institution's subscription for 90 days from any location. You must be logged in while authenticated at least once every 90 days to maintain this remote access.


About MyAccess

If your institution subscribes to this resource, and you don't have a MyAccess profile, please contact your library's reference desk for information on how to gain access to this resource from off-campus.

Subscription Options

AccessPediatrics Full Site: One-Year Subscription

Connect to the full suite of AccessPediatrics content and resources including 20+ textbooks such as Rudolph’s Pediatrics and The Pediatric Practice series, high-quality procedural videos, images, and animations, interactive board review, an integrated pediatric drug database, and more.

$595 USD
Buy Now

Pay Per View: Timed Access to all of AccessPediatrics

24 Hour Subscription $34.95

Buy Now

48 Hour Subscription $54.95

Buy Now

Pop-up div Successfully Displayed

This div only appears when the trigger link is hovered over. Otherwise it is hidden from view.