TY - CHAP M1 - Book, Section TI - Chapter 313. Human Herpesvirus 8 A1 - Schleiss, Mark R. A2 - Rudolph, Colin D. A2 - Rudolph, Abraham M. A2 - Lister, George E. A2 - First, Lewis R. A2 - Gershon, Anne A. Y1 - 2011 N1 - T2 - Rudolph's Pediatrics, 22e AB - The history of the identification of human herpesvirus 8 (HHV-8; also known as Kaposi sarcoma herpesvirus [KSHV] or Kaposi sarcoma [KS] virus) was unique among the Herpesviridae insofar as the virus was initially “discovered” purely on a molecular biologic basis using a powerful detection technique.1 Working with tissue from HIV- infected individuals with KS, Chang and colleagues used a technique referred to as “representational difference analysis” to identify disease-specific DNA sequences in KS tissue. On DNA sequence analysis, the deduced amino acid sequences were found to have strong homology to proteins from the gamma herpesvirus subfamily, the subfamily of the Herpesviridae that includes Epstein-Barr virus (EBV). This observation was striking in view of the known ability of EBV to persist in lymphocytes, immortalize cells, and produce human malignancies (Burkitt lymphoma and nasopharyngeal carcinoma). Hence, the novel gamma herpesvirus, HHV-8, appeared to be a new herpesvirus associated with human malignancy, Kaposi sarcoma. Eventually HHV-8 was cultivated in tissue culture, proving that these DNA sequences corresponded to a morphologically identifiable viral particle.2 SN - PB - The McGraw-Hill Companies CY - New York, NY Y2 - 2024/03/29 UR - accesspediatrics.mhmedical.com/content.aspx?aid=7030117 ER -